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Regulatory effect of HDCF on the mitochondrial apoptosis pathway in Ang II-induced HUVECs. (A) Representative immunoblotting images of ASK1, caspase 9, cleaved-caspase 3, Bcl-2, and Bax. (B) Relative expression levels of ASK1. (C) Relative expression level of caspase 9. (D) Relative expression level of cleaved-caspase 3. (E) Relative expression level of Bcl-2. (F) Relative expression level of Bax. Data are expressed as mean ± SD (n = 6). Different lowercase letters above the bars in the histogram indicate significant differences between treatment groups at P < 0.05.

Journal: Ultrasonics Sonochemistry

Article Title: Unlocking the potential of garlic by-product protein: Ultrasound-induced structural modification and fabrication of an ACE inhibitory peptide targeting endothelial dysfunction

doi: 10.1016/j.ultsonch.2025.107642

Figure Lengend Snippet: Regulatory effect of HDCF on the mitochondrial apoptosis pathway in Ang II-induced HUVECs. (A) Representative immunoblotting images of ASK1, caspase 9, cleaved-caspase 3, Bcl-2, and Bax. (B) Relative expression levels of ASK1. (C) Relative expression level of caspase 9. (D) Relative expression level of cleaved-caspase 3. (E) Relative expression level of Bcl-2. (F) Relative expression level of Bax. Data are expressed as mean ± SD (n = 6). Different lowercase letters above the bars in the histogram indicate significant differences between treatment groups at P < 0.05.

Article Snippet: Antibodies against Keap1, Nrf2, ASK1, and cleaved-caspase-3 were obtained from Proteintech Group, Inc. (Wuhan, China).

Techniques: Western Blot, Expressing